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KMID : 0545120020120010137
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 1 p.137 ~ p.141
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Bioaccumulation of Chromium Ions by Immobilized Cells of a Filamentous Cyanobacterium, Anabaena variabilis
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Khattar, Jasvir I. S.
Sarma, Tangirala A./Singh, Davinder P./Sharma, Anuradha
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Abstract
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Anabaena variabilis ATCC 29413 grew in chromium (Cr) containing Chu-10 (basal) and nitrate-supplemented media, and the growth of the organism in 100μM chromium was found to be 50% of that in control medium. The growth in nitrate (NO_3^-) supplemented cultures was better as compared to cultures grown in basal medium. Free cells from basal and nitrate-supplemented media removed 5.2 and 7.4n ㏖ of chromium ㎎^-1 protein in 8 h, respectively, from the medium containing 30μm chromium. The efficiency of chromium removal increased 7-fold in imidazole buffer (0.2 M, pH 7.0). A cell density equivalent to 100 ㎍ protein ml^-1 was found to be optimum for maximum Cr removal. Entrapment of cells in calcium-alginate beads did not affect the rate of Cr uptake by the cells. The efficiency of the laboratory-scale continuous flow bioreactor (12.5×2㎝) loaded with alginate-immobilized cells (10 ㎎ protein) and fed with 30μM chromium solution was compared at different flow rates. The efficiency of the bioreactor varied with flow rates. In terms of percent removal of Cr from influent, a flow rate of 0.1 ml min^-1 was found to be optimum for 6 h (54% Cr removal efficiency). Maximum amount of Cr (883 n㏖) was removed by the cells in 3 h at a flow rate of 0.5 ml min^-1. The potential use of A. variabilis in removing Cr from industrial effluents is discussed.
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